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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two supplemental splice variants have been described but comprehensive nucleotide sequence information and facts will not be but out there.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded through the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with check here and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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